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Textile Microbiology Testing



Standard Test Number Details of Test Conducted

AATCC 147*

Antibacterial activity assessment of textile materials: Parallel Streak Method


This is a qualitative test to detect antibacterial activity of diffusible antimicrobial agents on treated textile materials. This is a widely used method for initial screening of bacteriostatic activity shown by different antimicrobial agents applied on textile materials.

AATCC 100*

Assessment of Antibacterial Finishes on Textiles Materials


AATCC 100 antimicrobial test method is a quantitative method for the evaluation of the degree of antibacterial activity of antibacterial finishes on textile material.

Microorganisms used are: Staphylococcus aureus and Klebsiella pneumoniae. Other microorganisms can also used upon request.

AATCC 30 Test 2*

Antifungal activity, assessment on textile materials: Mildew and Rot resistance of Textile materials Test-II – Agar Plate, Chaetomium globosum


This test is used for evaluating rot resistance of cellulose containing textile materials that will not come in contact with soil.

AATCC 30 Test 3*

Antifungal activity, assessment on textile materials: Mildew and Rot resistance of Textile materials Test-III – Agar Plate, Aspergillus niger


This test evaluates the growth of Aspergillus niger on textile specimens. The textile specimens are kept on the surface of an inoculated agar plate and incubation is carried out for 7 days.

AATCC 30 Test 4

Antifungal activity, assessment on textiles materials: Mildew and Rot Resistance of Textile materials Test –IV – Humidity Jar, Mixed spore suspension


The test determines the fungistatic effectiveness of treatments intended to control mildew and non-pathogenic fungal growth on articles or surfaces composed of textile materials intended for outdoor and above ground level use and which are usually water-proofed.

AATCC 174*

Antimicrobial Activity Assessment of New Carpets


  1. AATCC 174 Part-1: A qualitative antibacterial assessment.
  2. AATCC 174 Part-2: A quantitative antibacterial assessment.
  3. AATCC 174 Part-3: A quantitative antifungal assessment.

This test method is designed to determine the antimicrobial activity of new carpet materials and consists of three procedures as mentioned above.


Antibacterial Activity of Fabrics, Detection of: Agar Plate Method


This test method is used for the detection of bacteriostatic activity of fabrics using Agar Plate Method which depends on diffusion of the antibacterial agent through agar.

JIS L 1902*

Test for antibacterial activity and efficacy on Textile Products


This test method is a quantitative method (Absorption Method) for testing antibacterial property of fabrics.
It is designed to evaluate the Antibacterial activity of fabrics treated with antibacterial finish to prevent microbial growth and to kill microorganisms over a specific contact period.

JIS Z 2801

Antimicrobial products - Test for antimicrobial activity and efficacy for plastics and other antimicrobial coated hard surfaces. (Film Contact Test Method)


The JIS Z2801 method is quantitative test method to determine the antimicrobial efficacy and activity of hard surfaces of Plastics, Metals, Glass, ceramic, rubber, silicone and other non absorbent materials.

ISO 20645*

Textile fabrics – Determination of antibacterial activity – Agar Diffusion Plate Test


This test is a qualitative method to determine the effect of diffusible antibacterial finishes applied to woven, knitted and other flat textiles.

ISO 20743*

Textiles – Determination of antibacterial activity of antibacterial finished products


This test method is a quantitative determination (Absorption Method) of the antibacterial activity of antibacterial finished textile products.

ASTM E 2149*

Determination of the Antimicrobial Activity of Immobilized Antimicrobial Agents Under Dynamic Contact Conditions


The ASTM E2149 test method is designed to evaluate the antimicrobial activity of non-leaching  antimicrobials treated specimens under dynamic contact conditions. This test method ensures good contact between the bacteria and the treated specimen by constant agitation of the test specimen in a challenge suspension during the test period.


Standard Practice for Determining Resistance of Synthetic Polymeric Materials to Fungi


This test method determines the effect of fungi on the properties of synthetic polymeric materials in the form of molded and fabricated articles, tubes, rods, sheets and film materials.

In-house Test Method

Evaluation of Bacterial Filtration Efficiency of Medical textiles


This test method is a quantitative method to measure the bacterial filtration efficiency (BFE) of medical face masks materials employing a ratio of the upstream bacterial challenge to downstream residual concentration.

IS 1622 & IS 5403*







Microbiological Examination of Water*


·         Total bacterial count (IS 1622) - Total bacterial count of water is estimated by performing standard plate count method, which is a direct measurement of the viable aerobic and facultative anaerobic bacteria.

·         Detection of coliforms (IS 1622) - Coliforms are detected in water by MPN Method.

·         Detection of E.coli (IS 1622) - E. coli are detected using Brilliant Green Bile Lactose Broth

·         Total fungal count (IS 5403) - Total Fungal count of water is estimated by determining viable yeast and mold count.

IS 1389

Methods for testing cotton fabrics for resistance to attack by microorganism by Humidity Chamber Method


The humidity chamber method is intended for determining qualitatively the susceptibility of the fabric to the growth of microorganisms associated with attack on cellulose.

ISO 11737 – Part I

Determination of a population of microorganisms on products


This test standard provides guidance for the enumeration and microbial characterization of the population of viable microorganisms on or in a medical device, component, raw material or package.

Bacteriological Analytical Manual

Aerobic Plate count & Yeast and Mold count


Aerobic plate count & Yeast and mold count is intended to indicate the level of total bacterial and fungal count respectively in a product.

* = Tests are under NABL scope

New Tests


Standard Test Number

Details of Test Conducted

ASTM E 2471

Standard Test Method for Using Seeded-Agar for the Screening Assessment of Antimicrobial Activity In Carpets


This test method qualitatively evaluates the presence of antibacterial and antifungal activity in or on carpets. ASTM E 2471 is used to assess the durability of the antimicrobial treatments on new carpets, and on those repeatedly shampooed or exposed to in-use conditions.

ASTM C 1338

Standard Test Method for Determining Fungi Resistance of Insulation Materials and Facings


This test method determines the relative ability of an insulation and its facing to support or resist fungal growth under conditions favorable for their development.

ASTM D 2020

Method A

Standard Test Methods for Mildew (Fungus) Resistance of Paper and Paperboard


This method covers the qualitative determination of mildew (fungus) resistance of paper and paperboard, particularly which have been given a fungus resistant treatment.

ASTM D 2574

Standard Test Method for Resistance of Emulsion Paints in the Container to Attack by Microorganisms


This test method covers the determination of the relative resistance of treated emulsion paints to attack in the container by organisms.

ISO 22196

Measurement of antibacterial activity on plastic surfaces


This test method is used for evaluating the antibacterial activity of treated plastic products.

ASTM D 495

Standard Specification for Retro-reflective Sheeting for Traffic Control


Fungus resistance of retro-reflective sheeting can be tested as per this method.